Description
RP2-Serpin-B11 is a polyclonal antibody made to the serine proteinase inhibitor serpin-B11. The antibody is made to a synthetic peptide based on the reactive center loop area of human serpin-B11. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.
Use
Serpin-B11 is a serine proteinase inhibitor of the ovalbumin-like B clade of serpins. Serpin-B11 was first discovered in human lung and prostate. Little is known about serpin-B11 tissue distribution and function. Most of the serpins (SERine Proteinase Inhibitors) are serine proteinase inhibitors; although not all serine proteinase inhibitors are serpins, and visa versa. The overall structural shape of the B clade of serpins most resembles ovalbumin, which is not considered to be a serine proteinase inhibitor. The serpins share a reactive center loop motif: a substrate bait loop which, when cleaved, confers a remarkable molecular shift in the serpin molecule, and traps the enzyme. This mousetrap method works because the uncleaved serpin molecule is in tension, and cleaving the loop allows a more energetically favorable state to exist. The cleavage site in serpin-B11 is Lys357-Ser358 of the 392 amino acid sequence. The original sequence described was 392 amino acids in length, with predicted mass of 44.1 kDa and pI of 8.62 Splice variants of 305, 278 and 190 amino acids have been reported, with predicted masses of 33.97, 31.6 and 21.1 kDa respectively, and pIs of 8.11, 9.03 and 6.07 respectively. The 305 and 190 amino acid forms share the same aminoterminus as the 392 amino acid form. The 305 amino acid form has a deletion in residues 120-206, of the 392 amino acid form. The 190 amino acid form has a deletion in residues 57-259 relative to the long form. The 278 amino acid form starts at the third methionine, relative to the long form. All four forms contain the reactive center loop of the long form, but it is unknown if the shorter forms are active serpins. The predicted isoelectric points of serpinB11 are significantly more basic than the other B-clade serpins, and suggest a different localization or function for this serpin. RP2-Serpin-B11 recognizes all of the listed forms of serpinB11. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species FOR RESEARCH USE ONLY; NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 12 at -20C.