Triple Point Biologics' Metalloproteinase Inhibitors recombinant catalog covers the four vertebrate tissue inhibitors of metalloproteinases (TIMPs): TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMPs are endogenous, secreted glycoproteins that regulate extracellular matrix remodeling by forming tight 1:1 stoichiometric complexes with active matrix metalloproteinases (MMPs), thereby inhibiting their proteolytic activity. Each TIMP family member exhibits a distinct inhibition profile across MMP subtypes and carries additional MMP-independent functions — TIMP-2, for example, participates in the cell-surface activation of pro-MMP-2 via MT1-MMP. The four TIMPs have been extensively characterized in published studies of tumor invasion, fibrosis, cardiac remodeling, and angiogenesis, making them central reference proteins in extracellular proteolysis research.

All recombinant TIMPs in this collection are expressed in HEK293 cells to preserve disulfide-bond integrity and relevant post-translational modifications, and are supplied as carrier-free preparations suitable for enzymatic assays and cell-based work. Each recombinant protein is paired with a matching rabbit polyclonal antibody raised and validated in the same laboratory — a program Triple Point Biologics has maintained for proteinase-related targets since 1994. Antibody cross-reactivity with the corresponding recombinant standard is validated by Western blot; IHC reactivity against tissue sections is documented where data are available. This matched-pair format allows researchers to use the recombinant protein as a defined positive control in immunoassays developed or validated with the companion antibody.

Common applications for these recombinant TIMPs include MMP inhibition kinetics (IC₅₀ determination, Ki measurement), matched-pair inhibitor–substrate experiments alongside catalog MMPs, and use as quantitative reference standards in cancer and cardiovascular biomarker studies. For guidance on configuring fluorogenic or chromogenic MMP activity assays with TIMP inhibitor controls, see the Protease Activity Assay Guide. For recommended concentrations and dilution strategies when using recombinant proteins as immunoassay positive controls, refer to the Recombinant Protein Positive Controls Guide. All products are for research use only.