Triple Point Biologics' Active Enzymes recombinant catalog covers proteins supplied in a confirmed catalytically active form — either as mature processed species or as zymogens that undergo auto-activation under defined assay conditions. Enzymes in this collection span proteases, kinases, phosphatases, and related classes whose activity is central to signal transduction, extracellular matrix remodeling, apoptosis, and immune regulation. Each lot is characterized for specific activity prior to release, providing a defined functional baseline for downstream experimental use. Activity is reported as units per milligram under stated substrate and buffer conditions, enabling direct comparison across experiments and between laboratories.

Recombinants in this collection are expressed in HEK293 cells, a mammalian host that supports the post-translational processing — including propeptide removal, disulfide bond formation, and glycosylation — required for native-like catalytic competence in many enzyme classes. Each recombinant protein in the catalog is paired with a matched rabbit polyclonal antibody generated in the same laboratory against the same target, with immunogen coverage and species cross-reactivity documented per entry. These antibodies have been validated for Western blot and immunohistochemistry; predicted cross-reactivity to additional species is noted where sequence homology supports it. Triple Point Biologics has characterized proteinase and inhibitor antibodies since 1994, and that provenance informs the depth of annotation accompanying each paired reagent.

Primary applications for this collection include activity assays, inhibitor IC50 determination, and substrate cleavage studies under defined kinetic conditions. The availability of a matched antibody for each recombinant facilitates parallel detection of endogenous protein in cell or tissue lysates alongside functional work with the recombinant form — a common requirement when validating inhibitor selectivity or confirming substrate identity. For protocol guidance on setting up activity measurements, see the Protease Activity Assays guide. For use of recombinant proteins as positive controls in immunoassay panels, refer to the Recombinant Protein Positive Controls guide.