DPP-10 (Recombinant)
- Expression system
- HEK293
- Cat. #
- REC-DPP10
In stock
- SKU
- REC-DPP10
Target Overview
DPP-10 (UniProt Q8N608; gene DPP10) is a 796-amino acid single-pass type II transmembrane protein and member of the dipeptidyl peptidase IV (DPPIV) family. Despite its structural homology to serine proteases, DPP-10 is catalytically inactive and carries no dipeptidyl aminopeptidase activity. Its primary characterised function is as an auxiliary subunit of voltage-gated potassium channels: DPP-10 promotes cell-surface trafficking of KCND2 (Kv4.2) and substantially modulates the gating kinetics, inactivation rates, and recovery properties of the resulting channel complex. This recombinant is produced by transient expression in HEK293 cells, a mammalian system that supports the glycosylation and folding environment appropriate for a membrane-associated auxiliary subunit. The full-length sequence spans residues 1–796 and encompasses the short cytoplasmic N-terminal region, the single transmembrane anchor, and the large extracellular/luminal DPPIV-homology domain that mediates channel interaction. In the laboratory, this recombinant serves several practical purposes. Researchers use it as a positive-control antigen in Western blot and immunohistochemistry experiments, particularly when validating antibodies raised against DPP-10 epitopes. It is also employed in co-immunoprecipitation and pull-down assays designed to map the interaction interface between DPP-10 and Kv4 channel subunits or the KChIP accessory proteins. Binding assays (surface plasmon resonance, bio-layer interferometry) using the purified extracellular domain fragment are a further described application. Researchers investigating neuronal or cardiac Kv4 channel complexes find the recombinant useful as a defined reference material. For groups validating antibody reagents against DPP-10, this recombinant can be paired directly with the matched Triple Point Biologics antibody (SKU: RP-DPP10).
Background
Applications
- Cell-surface trafficking assay: quantifying DPP-10-dependent promotion of KCND2 expression at the plasma membrane in transfected HEK293 or CHO cells
- Co-immunoprecipitation / pull-down: mapping the DPP-10 interaction interface with KCND2 or KChIP auxiliary subunits using purified recombinant as bait or prey
- Antibody validation positive control: spike-in or recombinant-loaded lane standard for Western blot validation of anti-DPP-10 antibodies, including the matched RP-DPP10 antibody
- Binding kinetics measurement: surface plasmon resonance or bio-layer interferometry determination of DPP-10 extracellular domain affinity for Kv4-family subunit fragments
- ELISA standard curve generation: quantification of endogenous DPP-10 in tissue lysates or conditioned media using recombinant as a calibrator
- Immunohistochemistry validation standard: cryo-section or FFPE control material for optimising anti-DPP-10 IHC staining protocols in neural or adipose tissue
- Structural/biophysical characterisation: circular dichroism, SEC-MALS, or HDX-MS studies of the DPPIV-homology domain fold and thermostability
References
- Sun W et al. Revealing Causal Protein Biomarkers and Potential Therapeutic Targets for Histologic-Specific Lung Cancer. J Cell Mol Med. 2025. doi:10.1111/jcmm.70866. PMID: 41340014.
- Whytock KL et al. Identification of a New Transcriptional Signature of Pre-Adipocytes in Human Subcutaneous White Adipose Tissue. Obesity (Silver Spring). 2026. doi:10.1002/oby.70135. PMID: 41633955.
- Guo Y et al. Semaglutide treatment in MOSH is associated with altered DNA methylation patterns of genes related to glycolipid metabolism. Sci Rep. 2026. doi:10.1038/s41598-026-50299-3. PMID: 42336886.
- Kim YA et al. Ethnic-Specific and UV-Independent Mutational Signatures of Basal Cell Carcinoma in Koreans. Int J Mol Sci. 2025. doi:10.3390/ijms26146941. PMID: 40725190.
- Wang D et al. Exploring the functional variations of key candidate genes affecting egg production by hypothalamic-pituitary-ovarian axis in chickens. Poult Sci. 2025. doi:10.1016/j.psj.2025.105027. PMID: 40132314.
Additional Specifications
| Storage Buffer | 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol |
|---|---|
| Endotoxin Level | <0.1 EU/µg by LAL |
| Purity (%) | >90% by SDS-PAGE |
| Expression System | HEK293 |
| Subcellular Localization | Subcellular localization not yet annotated |
Frequently Asked Questions
What is the expected molecular weight of recombinant DPP-10 on SDS-PAGE or Western blot?
The full-length DPP-10 recombinant (residues 1–796) has a predicted molecular weight of ~90 kDa from sequence alone, but expect an apparent band between 95–105 kDa on reducing SDS-PAGE due to N-linked glycosylation acquired during HEK293 expression. Purity is >90% by SDS-PAGE. If you are running this alongside native lysate, note that endogenous DPP-10 can run anomalously due to glycosylation heterogeneity; the recombinant band is typically sharper and more discrete than the lysate smear.
Does recombinant DPP-10 have any dipeptidyl peptidase catalytic activity I should account for in my assay?
No. DPP-10 is catalytically inactive despite its structural homology to the serine protease DPPIV family. The active-site residues required for dipeptidyl aminopeptidase activity are not conserved in DPP-10, and no substrate cleavage has been reported for this protein. If you are screening for DPP4/DPP8/DPP9 activity and using DPP-10 as a selectivity counter-screen, this recombinant is appropriate for that purpose — you should expect no turnover of standard substrates such as Gly-Pro-AMC under any assay condition.
What is the primary functional role of DPP-10 recombinant protein for voltage-gated potassium channel assays?
DPP-10 functions as an auxiliary subunit that physically associates with KCND2 (Kv4.2) and related Kv4-family channels, accelerating cell-surface trafficking and modulating gating kinetics including inactivation rate and recovery from inactivation. In reconstituted channel complex experiments, DPP-10 (residues 1–796) provides the full extracellular DPPIV-homology domain responsible for channel interaction. For co-immunoprecipitation or pull-down approaches, co-expressing or co-incubating this recombinant with Kv4.2 protein is the validated use case. Concentrations of 0.5–2 µg per reaction are a reasonable starting point for binding assays.
What buffer is recombinant DPP-10 supplied in and how should I dilute it for channel interaction assays?
DPP-10 recombinant is supplied in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol. This buffer is compatible with most downstream co-precipitation and SPR binding setups without exchange. For functional reconstitution assays where glycerol may interfere with lipid bilayer preparations, dilute at least 1:5 into your working buffer to reduce glycerol below 2%. Avoid diluting below ~0.05 µg/µL in low-protein buffers without adding a carrier such as 0.1% BSA, as membrane-associated proteins are prone to adsorptive losses at very low concentrations.
How should I store recombinant DPP-10 and what is its shelf life after aliquoting?
Store at -20°C in single-use aliquots immediately upon receipt. Repeated freeze-thaw cycles will degrade activity and promote aggregation of this membrane-associated auxiliary subunit. Properly stored aliquots are stable for at least 12 months from the date of manufacture. Once thawed, keep on ice and use within the same working day; do not refreeze. The 10% glycerol in the storage buffer acts as a cryoprotectant. There is no need to add additional glycerol, but do not dilute the stock and then refreeze the remainder.
Can I use recombinant DPP-10 as a positive control for Western blot with the matched antibody RP-DPP10?
Yes — this is a primary intended use. The matched rabbit polyclonal antibody RP-DPP10 (/anti-dpp-10-rabbit-polyclonal-antibody) was raised and validated against DPP-10 protein from the same expression system, making this recombinant the most direct positive control available for Western blot. Run 50–200 ng of recombinant DPP-10 per lane alongside your experimental lysate. Expect a band at ~95–105 kDa. The recombinant band confirms antibody sensitivity and confirms the gel region where endogenous DPP-10 should appear in your tissue or cell lysate lanes.
How much recombinant DPP-10 should I load per lane for a Western blot positive control?
A loading range of 50–200 ng per lane works reliably with the matched antibody RP-DPP10 under standard reducing SDS-PAGE conditions. Start at 100 ng for initial optimization; this typically yields a clean band at ~95–105 kDa with the RP-DPP10 antibody at 1:500–1:2000 dilution. If your secondary antibody has high background, reduce recombinant loading to 50 ng rather than diluting primary further. Endotoxin is <0.1 EU/µg by LAL, so this preparation is also suitable for cell-based loading controls where endotoxin contamination would confound results.
Is recombinant DPP-10 validated for use in co-immunoprecipitation experiments targeting the Kv4.2 channel complex?
DPP-10 (residues 1–796, HEK293-expressed) is appropriate for co-IP and pull-down experiments with Kv4.2/KCND2, as it contains the complete extracellular DPPIV-homology domain that mediates channel association. For antibody-based co-IP validation, RP-DPP10 has been validated for Western blot detection and can be used to confirm DPP-10 presence in the pulled-down complex. Recommended starting input: 1–2 µg recombinant DPP-10 per co-IP reaction. Include a no-bait negative control. Glycerol in the storage buffer does not interfere with protein A/G bead-based pull-down protocols at standard input volumes.
Validation imagery coming soon
Western blot validation figures for REC-DPP10 will be published here as they are produced in-house.
If you would like to see existing validation data for this antibody before publication, request a sample copy.