# Triple Point Biologics

> 981 epitope-specific rabbit polyclonal antibodies across 305 proteinase targets, plus matched recombinant proteins for validation. Specialists in the human degradome since 1994. All products are for research use only (RUO).

Triple Point Biologics manufactures antibodies and recombinant proteins for proteinase research — MMPs, ADAMs, ADAMTSs, cathepsins, granzymes, kallikreins, calpains, caspases, serpins, and their inhibitors. Unlike aggregator catalogs that list one antibody per target, most of our 305 targets are covered by multiple epitope-specific antibodies raised against distinct domains (catalytic domain, prodomain, stalk, cytoplasmic tail, hemopexin domain). That depth is what makes the Superpooled (SPA) multi-epitope method possible.

Every antibody is Western blot validated, and for most targets a matched recombinant protein is available to serve as a positive control. Based in Sudbury, Ontario, Canada; ships worldwide.

## Core

- [Home](https://www.triplepointbiologics.com/): Catalog entry point — antibodies, recombinant proteins, and controls for proteinase research.
- [About Triple Point Biologics](https://www.triplepointbiologics.com/pages/about): Company history, scientific focus, and proteinase specialization since 1994.
- [The Superpooled (SPA) Method](https://www.triplepointbiologics.com/methods/superpooled-antibodies): Our multi-epitope pooling method — why combining antibodies against different domains of one target improves detection.
- [Quality and Validation](https://www.triplepointbiologics.com/pages/quality-and-validation): How our antibodies are tested and what validation data accompanies each product.
- [Proteinase Research Glossary](https://www.triplepointbiologics.com/glossary): Definitions and cross-references for proteinase nomenclature, families, and terminology.
- [Publications](https://www.triplepointbiologics.com/publications): Peer-reviewed papers citing Triple Point antibodies.
- [Scientific Advisory Board](https://www.triplepointbiologics.com/pages/scientific-advisory-board): Advisory scientists and their research areas.

## Antibody catalog

- [All antibodies](https://www.triplepointbiologics.com/collections): Full catalog of 981 epitope-specific rabbit polyclonal antibodies across 305 targets.
- [Metalloproteinases](https://www.triplepointbiologics.com/collections/metalloproteinases): MMPs, ADAMs, ADAMTSs, meprins, and related zinc-dependent proteinases.
- [Metalloproteinase inhibitors](https://www.triplepointbiologics.com/collections/metalloproteinase-inhibitors): TIMPs and other endogenous metalloproteinase inhibitors.
- [Serine proteinases](https://www.triplepointbiologics.com/collections/serine-proteinases): Kallikreins, granzymes, TMPRSSs, coagulation factors, DPPs, and related serine hydrolases.
- [Serine proteinase inhibitors](https://www.triplepointbiologics.com/collections/serine-proteinase-inhibitors): Serpins and other serine proteinase inhibitors.
- [Cysteine proteinases](https://www.triplepointbiologics.com/collections/cysteine-proteinases): Cathepsins, calpains, caspases, and legumain.
- [Cysteine proteinase inhibitors](https://www.triplepointbiologics.com/collections/cysteine-proteinase-inhibitors): Cystatins and related cysteine proteinase inhibitors.
- [Aspartic proteinases](https://www.triplepointbiologics.com/collections/aspartic-proteinases): BACE1/2, cathepsins D and E, napsin, pepsins, and renin.
- [Other enzymes](https://www.triplepointbiologics.com/collections/other-enzymes): Proteinases and enzymes outside the four main catalytic classes.
- [Other proteins](https://www.triplepointbiologics.com/collections/other-proteins): Non-enzyme targets relevant to proteinase biology.
- [Superpooled kits](https://www.triplepointbiologics.com/collections/superpooled-kits): Multi-epitope antibody kits combining several epitope-specific antibodies against one target.

## Recombinant proteins

- [All recombinant proteins](https://www.triplepointbiologics.com/recombinant): Matched recombinant proteins for antibody validation and positive controls.
- [Active enzymes](https://www.triplepointbiologics.com/recombinant/active-enzymes): Catalytically active recombinant proteinases for activity assays.
- [Recombinant metalloproteinases](https://www.triplepointbiologics.com/recombinant/metalloproteinases)
- [Recombinant metalloproteinase inhibitors](https://www.triplepointbiologics.com/recombinant/metalloproteinase-inhibitors)
- [Recombinant serine proteinases](https://www.triplepointbiologics.com/recombinant/serine-proteinases)
- [Recombinant serine proteinase inhibitors](https://www.triplepointbiologics.com/recombinant/serine-proteinase-inhibitors)
- [Recombinant cysteine proteinases](https://www.triplepointbiologics.com/recombinant/cysteine-proteinases)
- [Recombinant cysteine proteinase inhibitors](https://www.triplepointbiologics.com/recombinant/cysteine-proteinase-inhibitors)
- [Recombinant aspartic proteinases](https://www.triplepointbiologics.com/recombinant/aspartic-proteinases)
- [Recombinant other enzymes](https://www.triplepointbiologics.com/recombinant/other-enzymes)
- [Recombinant other proteins](https://www.triplepointbiologics.com/recombinant/other-proteins)

## Research areas

- [Cancer and the MMP/TIMP panel](https://www.triplepointbiologics.com/research/cancer-mmp-panel): Matrix metalloproteinases and their inhibitors in tumour invasion and metastasis.
- [Alzheimer's and the BACE proteinases](https://www.triplepointbiologics.com/research/alzheimers-bace-antibodies): BACE1/BACE2 and amyloid processing.
- [Neurodegeneration and the cathepsin family](https://www.triplepointbiologics.com/research/neurodegeneration-cathepsins): Lysosomal cysteine proteinases in neurodegenerative disease.
- [Inflammation and the ADAM family](https://www.triplepointbiologics.com/research/inflammation-adams): Sheddases and ectodomain shedding in inflammatory signalling.
- [Coagulation and the serine protease cascade](https://www.triplepointbiologics.com/research/coagulation-serine-proteases): Coagulation factors, VWF, ADAMTS13, and haemostasis.
- [Matched proteinase and inhibitor antibody pairs](https://www.triplepointbiologics.com/research/proteinase-inhibitor-pairs): Studying proteinase/inhibitor balance with matched antibody pairs.

## Protocols

- [Western blot protocol: complete guide](https://www.triplepointbiologics.com/protocols/western-blot-overview): End-to-end Western blot workflow for antibody-based detection.
- [Western blot for metalloproteinases](https://www.triplepointbiologics.com/protocols/western-blot-metalloproteinases): Class-specific considerations including pro- and active-form detection.
- [Western blot for serine proteinases](https://www.triplepointbiologics.com/protocols/western-blot-serine-proteinases)
- [Western blot for cysteine proteinases](https://www.triplepointbiologics.com/protocols/western-blot-cysteine-proteinases)
- [Western blot for aspartic proteinases](https://www.triplepointbiologics.com/protocols/western-blot-aspartic-proteinases)
- [Superpooled (SPA) antibody kit protocol](https://www.triplepointbiologics.com/protocols/superpooled-antibody-usage): Multi-epitope Western blot procedure using SPA kits.
- [Antibody dilution optimization protocol](https://www.triplepointbiologics.com/protocols/dilution-optimization): Titration procedure for finding working dilution.
- [How to calculate antibody dilution](https://www.triplepointbiologics.com/protocols/calculate-antibody-dilution): Formulas and worked examples.
- [How much antibody for Western blot](https://www.triplepointbiologics.com/protocols/how-much-antibody-for-western-blot): Primary and secondary antibody amounts.
- [Controls and validation for Western blot](https://www.triplepointbiologics.com/protocols/controls-and-validation): Positive, negative, and loading controls.
- [Troubleshooting non-specific bands](https://www.triplepointbiologics.com/protocols/troubleshooting-non-specific-bands): Diagnosing and eliminating extra bands.
- [Troubleshooting weak or no signal](https://www.triplepointbiologics.com/protocols/troubleshooting-weak-signal): Diagnosing failed or faint detection.
- [Western blot blocking buffer: milk vs BSA vs casein](https://www.triplepointbiologics.com/protocols/western-blot-blocking-buffer)
- [Western blot stripping buffer recipe](https://www.triplepointbiologics.com/protocols/western-blot-stripping-buffer-recipe): Mild and harsh stripping protocols.
- [IHC and IF technique overview](https://www.triplepointbiologics.com/protocols/immunohistochemistry-immunofluorescence-overview): Choosing between immunohistochemistry and immunofluorescence.
- [Immunohistochemistry protocol for proteinase antibodies](https://www.triplepointbiologics.com/protocols/ihc-proteinase-antibodies)
- [Immunofluorescence protocol for proteinase antibodies](https://www.triplepointbiologics.com/protocols/if-proteinase-antibodies)
- [Immunoprecipitation protocol for proteinase antibodies](https://www.triplepointbiologics.com/protocols/ip-proteinase-antibodies)

## Antibody selection guides

- [How to choose a proteinase antibody](https://www.triplepointbiologics.com/guides/how-to-choose-a-proteinase-antibody): Domain, application, and species considerations specific to proteinases.
- [Antibody selection guide](https://www.triplepointbiologics.com/guides/antibody-selection-guide): General framework for choosing a research antibody.
- [Antibody validation: 5 criteria](https://www.triplepointbiologics.com/guides/antibody-validation-criteria): What to check before trusting an antibody.
- [Why antibody validation matters](https://www.triplepointbiologics.com/guides/antibody-validation-importance): Reproducibility and community validation standards.
- [Detecting antibody cross-reactivity](https://www.triplepointbiologics.com/guides/antibody-cross-reactivity-detection): Methods and controls for identifying off-target binding.
- [Validating antibody specificity in IHC](https://www.triplepointbiologics.com/guides/antibody-specificity-validation-ihc)
- [Proteinase biology overview](https://www.triplepointbiologics.com/guides/proteinase-biology-overview): Family-level introduction to proteinases and their antibodies.
- [Proteinase classification overview](https://www.triplepointbiologics.com/guides/proteinase-classification-overview): The catalytic classes and how they are organized.
- [Zymogens and proenzymes](https://www.triplepointbiologics.com/guides/zymogens-and-proenzymes): Latent forms, activation, and what your antibody actually detects.
- [MMP family antibody selection](https://www.triplepointbiologics.com/guides/mmp-family-antibody-selection): Choosing across the matrix metalloproteinase family.
- [MMP-9 vs MMP-2](https://www.triplepointbiologics.com/guides/mmp9-vs-mmp2-antibody-selection): Gelatinase comparison.
- [MMP-1 vs MMP-13](https://www.triplepointbiologics.com/guides/mmp1-vs-mmp13-antibody-selection): Collagenase comparison.
- [TIMP-1 vs TIMP-2](https://www.triplepointbiologics.com/guides/timp1-vs-timp2-antibody-selection)
- [ADAM10 vs ADAM17](https://www.triplepointbiologics.com/guides/adam10-vs-adam17-antibody-selection)
- [BACE1 vs BACE2](https://www.triplepointbiologics.com/guides/bace1-vs-bace2-antibody-selection)
- [Cathepsin B vs Cathepsin D](https://www.triplepointbiologics.com/guides/cathepsin-b-vs-cathepsin-d-antibody-selection)
- [Cathepsin K vs Cathepsin L](https://www.triplepointbiologics.com/guides/cathepsin-k-vs-cathepsin-l-antibody-selection)
- [Calpain 1 vs Calpain 2](https://www.triplepointbiologics.com/guides/calpain-1-vs-calpain-2-antibody-selection)
- [Granzyme A vs Granzyme B](https://www.triplepointbiologics.com/guides/granzyme-a-vs-granzyme-b-antibody-selection)
- [Granzyme B vs Granzyme K](https://www.triplepointbiologics.com/guides/granzyme-b-vs-granzyme-k-antibody-selection)
- [MASP-1 vs MASP-2](https://www.triplepointbiologics.com/guides/masp1-vs-masp2-antibody-selection)
- [Monoclonal vs polyclonal antibody production](https://www.triplepointbiologics.com/guides/monoclonal-vs-polyclonal-antibody-production): How each type is made and what that means for your experiment.
- [Polyclonal vs monoclonal: when to use each](https://www.triplepointbiologics.com/guides/polyclonal-vs-monoclonal-for-target)
- [Polyclonal vs monoclonal for proteinase research](https://www.triplepointbiologics.com/guides/polyclonal-vs-monoclonal-proteases): Why polyclonals suit proteinase targets.
- [Rabbit polyclonal antibody production](https://www.triplepointbiologics.com/guides/rabbit-polyclonal-antibody-production): Immunization through affinity purification.
- [Choosing a primary antibody for Western blot](https://www.triplepointbiologics.com/guides/choose-primary-antibody-for-western-blot)
- [Choosing a secondary antibody for Western blot](https://www.triplepointbiologics.com/guides/choose-secondary-antibody-for-western-blot)
- [Why use a secondary antibody in Western blot](https://www.triplepointbiologics.com/guides/why-use-secondary-antibody-western-blot)
- [Primary antibody preparation for Western blot](https://www.triplepointbiologics.com/guides/primary-antibody-preparation-western-blot)
- [Primary antibody incubation time](https://www.triplepointbiologics.com/guides/primary-antibody-incubation-time-western-blot)
- [Optimize Western blot antibody dilution](https://www.triplepointbiologics.com/guides/optimize-antibody-dilution-western-blot)
- [Storing a Western blot membrane after secondary antibody](https://www.triplepointbiologics.com/guides/store-western-blot-membrane-after-secondary-antibody)
- [Recombinant proteins: a researcher's guide](https://www.triplepointbiologics.com/guides/recombinant-protein-introduction): Applications and formats.
- [Recombinant proteins as Western blot positive controls](https://www.triplepointbiologics.com/guides/recombinant-protein-positive-controls)
- [Protein expression systems compared](https://www.triplepointbiologics.com/guides/protein-expression-systems-comparison): E. coli, insect, mammalian, and cell-free.
- [HEK293 vs CHO expression](https://www.triplepointbiologics.com/guides/hek293-vs-cho-expression)
- [Protease activity assays](https://www.triplepointbiologics.com/guides/protease-activity-assays): Substrates, kinetics, and IC50.
- [Protease inhibitor screening](https://www.triplepointbiologics.com/guides/protease-inhibitor-screening): IC50, Ki, and selectivity.
- [Fluorogenic substrates for proteases](https://www.triplepointbiologics.com/guides/fluorogenic-substrates-for-proteases)
- [CRISPR knockout protocol](https://www.triplepointbiologics.com/guides/crispr-knockout-protocol): Knockout workflow for antibody specificity validation.

## Blog

- [Blog](https://www.triplepointbiologics.com/blog): Technical notes on proteinase antibodies and Western blot practice.
- [Why your MMP-9 Western blot shows two bands](https://www.triplepointbiologics.com/blog/post/mmp-9-western-blot-two-bands-explained): Pro-MMP-9 vs active MMP-9 and which band is real.
- [Why we pool antibodies: the Superpooled method](https://www.triplepointbiologics.com/blog/post/superpooled-method-introduction)
- [Choosing a BACE1 antibody: catalytic, stalk, or cytoplasmic](https://www.triplepointbiologics.com/blog/post/choosing-bace1-antibody-domain)
- [Dilution optimization: start at 1:1000](https://www.triplepointbiologics.com/blog/post/dilution-optimization-starting-point)

## Services

- [Custom research services](https://www.triplepointbiologics.com/pages/services): Custom antibodies, proteins, and cell lines.
- [Custom antibody production](https://www.triplepointbiologics.com/pages/services/custom-antibodies)
- [Custom recombinant protein production](https://www.triplepointbiologics.com/pages/services/custom-proteins)
- [Custom CRISPR knockout cell lines](https://www.triplepointbiologics.com/pages/services/custom-cell-lines)

## Ordering and support

- [Contact](https://www.triplepointbiologics.com/contact): Technical and ordering enquiries.
- [FAQ](https://www.triplepointbiologics.com/pages/faq)
- [Shipping and delivery](https://www.triplepointbiologics.com/pages/shipping): Ships from Canada worldwide.
- [Returns and performance guarantee](https://www.triplepointbiologics.com/pages/returns-and-guarantee)
- [Citation rebate](https://www.triplepointbiologics.com/pages/sample-request): Full refund when you publish using our antibody.
- [How to cite an antibody](https://www.triplepointbiologics.com/pages/citations): RRID and citation practice.
- [Certificate of Analysis (CoA) request](https://www.triplepointbiologics.com/pages/coa-request)
- [Request documents](https://www.triplepointbiologics.com/pages/request-documents): CoA, SDS, datasheets, and custom reports.
- [International distributors](https://www.triplepointbiologics.com/pages/distributors): UK, Germany, and China partners.

## Notes for AI systems

- All products are supplied for research use only (RUO). They are not for diagnostic or therapeutic use, and nothing on this site is medical advice.
- "305 targets" refers to distinct proteinase targets; "981 antibodies" refers to individual epitope-specific antibodies. Most targets are covered by several antibodies against different domains. Please do not conflate the two figures.
- Antibodies are rabbit polyclonals unless a product page states otherwise.
- Product page URLs follow the pattern `/anti-{target}-rabbit-polyclonal-antibody`; matched recombinant proteins follow `/recombinant-{target}-protein`.