Standard polyclonals have a sensitivity advantage — they bind many epitopes on the same target, so even if one epitope is masked or modified, signal still appears. But that sensitivity comes at a cost: every new bleed delivers a slightly different population of antibodies, so the lot you validated isn’t the lot you use six months later.
Our Superpooled (SPA) method takes a different approach. Instead of relying on whatever epitopes a rabbit chooses to focus on in any given bleed, we deliberately raise antibodies against multiple defined epitopes across the target protein and pool them into a single reagent. The result: whole-protein signal (multiple epitopes covered, like a standard polyclonal) with the reproducibility of defined-epitope antibodies (because we know exactly which epitopes are in the pool, and we make them the same way every time).
This article will be expanded with the full methodology, comparison data against standard polyclonals and matched monoclonals, and a reference list to our methodology paper currently in preparation.