Anti-Serpin-A13 Rabbit Polyclonal Antibody

Frequently Asked Questions

What molecular weight band should I expect for Serpin-A13 on Western blot?

Serpin-A13 is predicted to encode a 307-amino acid secreted protein with an expected molecular weight around 35–38 kDa, depending on post-translational modifications. Because functional characterization remains limited and the gene is annotated as putative, published Western blot data are sparse. You may observe a single band near this range in lysates from tissues or cells expressing SERPINA13. If you detect multiple bands, consider glycosylation or cleavage products; serpins in the A-clade are often glycosylated. Run a positive control lysate known to express the transcript and include molecular-weight markers spanning 25–50 kDa.

Is Serpin-A13 functional or is it a pseudogene?

SERPINA13 is annotated as putative in UniProt (Q6UXR4), reflecting incomplete experimental validation of protease inhibitory activity or physiological substrates. The gene encodes a predicted 307-residue secreted protein with conserved serpin-fold features, but unlike well-characterized family members such as SERPINA1 or SERPINA3, direct evidence of enzymatic inhibition or in vivo function is limited in the literature. It is not classified as a pseudogene; transcripts have been detected in some tissues. Researchers should interpret expression data cautiously and consider functional assays—such as protease-inhibition screens—if exploring its biological role.

What dilution should I start with for Western blot with this Serpin-A13 antibody?

We recommend starting at 1:1000 dilution in blocking buffer for Western blot, based on our validation data. This polyclonal was raised in rabbit and supplied at 100 µg per vial. Optimal dilution can vary with your sample type, expression level, and detection system. If signal is weak, titrate up to 1:500; if background is high, extend to 1:2000. Serpin-A13 expression may be low or restricted, so enrich secreted fractions or use lysates from tissues where SERPINA13 transcripts are reported. Always include a no-primary-antibody control to assess non-specific binding.

Can I use this antibody for immunohistochemistry or immunofluorescence?

Triple Point Biologics antibodies are routinely validated for Western blot and immunohistochemistry; many also perform well in immunofluorescence. This Serpin-A13 antibody is a rabbit polyclonal, which typically works across multiple platforms. For IHC, start with antigen retrieval in citrate buffer (pH 6.0) and a 1:100–1:200 dilution; titrate as needed. For IF, try 1:50–1:100. Because Serpin-A13 is predicted to be secreted, expect extracellular or perinuclear Golgi staining if the protein is expressed. Validate specificity with a blocking peptide or siRNA knockdown if available, given limited published staining patterns for this target.

Does this Serpin-A13 antibody cross-react with other species besides human?

This antibody is validated for human Serpin-A13. Cross-reactivity with other species is not yet confirmed. Serpin-A13 sequence conservation across mammals is moderate; if you work with mouse or rat samples, homology may be sufficient for detection, but you should verify by Western blot in your model system. Run a side-by-side comparison with human lysate as a positive control and check for a band at the expected molecular weight. Predicted cross-reactivity is not a guarantee; empirical testing is essential. Contact us if you generate data in non-human species—we value user feedback for expanding validation.

What sample types are best for detecting Serpin-A13?

Because Serpin-A13 is predicted to be secreted, conditioned media, serum, or extracellular-matrix fractions may yield stronger signal than whole-cell lysates. Transcript-expression databases suggest restricted or low-level expression in certain tissues; confirm mRNA presence by qPCR if possible before committing to protein detection. For Western blot, concentrate conditioned medium by ultrafiltration or acetone precipitation to boost signal. If using tissue lysates, include protease inhibitors during extraction to preserve serpin integrity. Positive-control selection is critical: pilot several cell lines or tissue types and verify by RT-PCR before assuming a sample is truly positive.

How should I store this Serpin-A13 antibody and how long is it stable?

Store the antibody at −20 °C in small aliquots to avoid repeated freeze-thaw cycles, which can reduce titer and increase aggregation. Polyclonal antibodies supplied in glycerol-containing buffer are typically stable for at least 12 months under these conditions. For day-to-day use, keep a working aliquot at 4 °C for up to one month; do not leave at room temperature. Avoid prolonged exposure to light. If you observe particulate matter, centrifuge briefly before use. We do not recommend long-term storage at 4 °C for the stock vial. Refer to the datasheet supplied with your lot for any batch-specific guidance.

What controls should I include when using this Serpin-A13 antibody?

Include a no-primary-antibody control to assess non-specific secondary binding, particularly important for polyclonals. Because Serpin-A13 is putative with limited published data, a positive-control lysate verified by RT-PCR for SERPINA13 transcript is essential; do not assume ubiquitous expression. If available, use a blocking peptide corresponding to the immunogen to confirm band specificity. A negative control—such as a cell line or tissue documented to lack SERPINA13 mRNA—helps distinguish true signal from cross-reactivity with other serpins. Molecular-weight markers spanning 25–50 kDa and a housekeeping protein (actin, tubulin) for loading control complete a rigorous Western blot experiment.