Anti-Serpin-A11 Rabbit Polyclonal Antibody

Frequently Asked Questions

What molecular weight should I expect for Serpin-A11 on Western blot?

Serpin-A11 migrates at approximately 47–50 kDa on reducing SDS-PAGE, corresponding to the 422-amino-acid mature secreted protein. The predicted molecular weight from sequence is around 46 kDa, but glycosylation and post-translational modifications can shift the apparent size upward. Because Serpin-A11 is secreted, you will detect it robustly in conditioned medium, serum, or plasma samples. If working with cell lysates, expect lower signal unless the cell type actively secretes Serpin-A11. Always include a positive control such as human plasma or serum to confirm antibody performance.

What dilution should I start with for Western blot with this Serpin-A11 antibody?

We recommend starting at 1:1000 dilution in blocking buffer for Western blot, which has been validated for this rabbit polyclonal. Depending on your sample type and Serpin-A11 abundance, you may optimize between 1:500 and 1:2000. Secreted samples such as plasma, serum, or conditioned medium typically yield strong signal at 1:1000. For cell lysates from non-secretory lines, consider concentrating your sample or using a more concentrated antibody dilution around 1:500. Titre against your specific lysate or fluid to establish your optimal working concentration.

Is this Serpin-A11 antibody cross-reactive with mouse or rat samples?

This antibody is validated for human Serpin-A11. Cross-reactivity with mouse or rat has not been experimentally confirmed. Human SERPINA11 shares moderate sequence homology with rodent orthologs, so cross-reactivity is possible but not guaranteed. If you plan to work with mouse or rat samples, we recommend running a pilot blot with positive control human plasma alongside your rodent sample. Check for a band at the expected molecular weight and verify specificity with a blocking peptide or Serpin-A11 knockout lysate if available.

What sample types are best for detecting Serpin-A11?

Serpin-A11 is a secreted protein, so plasma, serum, and cell culture supernatants are the most reliable sample types. It has been detected in exosomal fractions as well. If using cell lysates, prioritize cell lines known to secrete Serpin-A11 or those relevant to cardiovascular and metabolic contexts where expression has been reported. For tissue work, consider organs with high secretory activity or vascular beds. Avoid whole-blood lysates unless you are specifically investigating circulating levels; plasma separation provides cleaner background and higher signal-to-noise.

Does Serpin-A11 have splice variants or isoforms I should be aware of?

Serpin-A11 is encoded by a single-exon mature coding sequence with limited evidence for functionally distinct splice isoforms in the literature. The canonical form is 422 amino acids after signal-peptide cleavage. Minor variants may exist at the transcript level, but they are not well characterized. This polyclonal antibody is raised against a large portion of the mature protein and should recognize the predominant circulating form. If you observe multiple bands on Western blot, consider post-translational modifications such as glycosylation or proteolytic processing rather than isoforms.

Can I use this antibody for immunohistochemistry or immunofluorescence?

Triple Point Biologics antibodies are routinely validated for Western blot and immunohistochemistry, and many perform well in immunofluorescence. This Serpin-A11 rabbit polyclonal has been validated for Western blot at 1:1000; IHC is supported across our product line. For IHC, start with 1:100 to 1:200 dilution and antigen retrieval in citrate buffer. For IF, begin at 1:50 to 1:100. Because Serpin-A11 is secreted, expect extracellular and perivascular staining in tissues. Validate specificity with a no-primary-antibody control and confirm expression pattern against known Serpin-A11–expressing tissues.

How should I store this Serpin-A11 antibody and what is its shelf life?

Store the antibody at –20 °C in single-use aliquots to avoid repeated freeze-thaw cycles, which can reduce titre and increase aggregation. The product is supplied at 100 µg and is stable for at least 12 months under these conditions. For short-term use, an aliquot can be kept at 4 °C for up to one month. Add sodium azide to a final concentration of 0.02–0.05 percent if storing at 4 °C for extended periods. Do not store diluted antibody; prepare working dilutions fresh in blocking buffer on the day of use.

What controls should I include when working with Serpin-A11 antibody?

Always include a positive control known to contain Serpin-A11, such as human plasma or serum, which will show a band at approximately 47–50 kDa. A negative control could be a cell line or tissue with negligible Serpin-A11 expression. If available, use a blocking peptide corresponding to the immunogen to confirm specificity; pre-incubate antibody with peptide and verify signal loss. For quantitative work or biomarker studies, run a dilution series of your positive control to assess linearity and dynamic range. Secondary-antibody-only lanes help rule out non-specific binding.