Anti-PACE-4 Rabbit Polyclonal Antibody
- Host
- Rabbit, Polyclonal
- Reactivity
- Human, Mouse, Rat
- UniProt
- P29122
- Size
- 100 µL
- Cat. #
- RP-PACE4
In stock
- SKU
- RP-PACE4
Rabbit polyclonal antibody against PACE-4 (PCSK6, Paired-basic Amino acid Cleaving Enzyme 4). PACE-4 is a member of the furin-like subtilisin/kexin family of proprotein convertases that cleave inactive precursors at basic residue motifs (R-X-K/R-R) to release biologically active proteins.
PACE-4 substrates include transforming growth factor-beta (TGF-β) family members, bone morphogenetic proteins (BMPs), vascular endothelial growth factor-C (VEGF-C), matrix metalloproteinases (MMPs), and integrin α subunits. Physiological roles include embryonic development (particularly craniofacial and skeletal patterning), cardiovascular biology, and cancer cell metastasis. PACE-4 overexpression drives tumor invasion in prostate and breast cancer through activation of MMPs and IGF-1R.
Applications: Western blot validated (RP1, N-terminal). Recommended positive controls: LNCaP prostate cancer lysate (endogenous overexpression), CHO or HEK293 cells transfected with human PCSK6, or SW480 colorectal cancer lysate. Additional TPB PACE-4 antibodies targeting the catalytic and C-terminal domains are in development.
Immunogen: Synthetic peptide corresponding to the N-terminal / propeptide region of human PACE-4 (UniProt P29122).
Reactivity: Human confirmed; mouse and rat predicted based on sequence identity (>90% in the immunogenic region).
Additional Specifications
| Size | 100 µg |
|---|---|
| Target Protein | PACE-4 (PCSK6) |
| UniProt ID | P29122 |
| Gene Symbol | PCSK6 |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to the N-terminal / propeptide region of human PACE-4 (UniProt P29122). |
| Conjugation | Unconjugated |
| Formulation | PBS, 0.02% sodium azide, 50% glycerol |
| Storage Conditions | -20°C long-term; 4°C short-term (≤1 month); avoid repeated freeze-thaw |
| Reactivity Species | Human |
| Validated Applications | Western Blot (WB) |
| Recommended Dilution | WB 1:1000 |
| Volume / Size | 100 µL |
| Proteinase Class | Serine Proteinase |
| Purity (%) | >95% (affinity purified) |
| Publication Citations | 0 |
| Alternate Names | Proprotein convertase subtilisin/kexin type 6 · Paired basic amino acid cleaving enzyme 4 · Subtilisin-like proprotein convertase 4 · SPC4 |
| References (HTML) |
|
| Potential Cross-Reactivity | Mouse, Rat |
Validation imagery coming soon
Western blot validation figures for PACE-4 (PCSK6) will be published here as they are produced in-house.
If you would like to see existing validation data for this antibody before publication, request a sample copy.
Also known as:
- Proprotein convertase subtilisin/kexin type 6
- Paired basic amino acid cleaving enzyme 4
- Subtilisin-like proprotein convertase 4
- SPC4
- UniProt Consortium. (2023) UniProt: the Universal Protein Knowledgebase in 2023. Nucleic Acids Res. 51(D1):D523–D531.
- Rawlings ND, Barrett AJ, Thomas PD, Huang X, Bateman A, Finn RD. (2018) The MEROPS database of proteolytic enzymes, their substrates and inhibitors in 2017. Nucleic Acids Res. 46(D1):D624–D632.
Help us track citations using this antibody — email us your DOI when your paper is accepted.