Anti-Kallistatin Rabbit Polyclonal Antibody
- Host
- Rabbit, Polyclonal
- Reactivity
- Human, Mouse, Rat
- UniProt
- P29622
- Size
- 100 µL
- Cat. #
- RP-Kallistatin
In stock
- SKU
- RP-Kallistatin
Rabbit polyclonal antibody against Kallistatin (SERPINA4). Kallistatin is a serpin-family serine protease inhibitor originally identified in human plasma as an inhibitor of tissue kallikrein (KLK1). Kallistatin regulates blood pressure through kinin signaling and has emerged as an angiogenesis regulator with important roles in cardiovascular disease, cancer progression, and inflammation.
Structurally, kallistatin is homologous to alpha-1 antitrypsin (SERPINA1) with a reactive center loop (RCL) that traps target proteases via covalent acyl-enzyme intermediates. Beyond its kallikrein-inhibiting activity, kallistatin exhibits protease-independent functions including anti-inflammatory signaling through the LRP6/Wnt pathway and inhibition of angiogenesis via binding to tropomyosin and interference with VEGF/eNOS signaling.
Three TPB antibody variants target N-terminal, reactive center loop, and C-terminal domains, enabling functional-state discrimination: N-terminal antibodies detect both native and cleaved kallistatin, while RCL-directed antibodies preferentially detect the intact inhibitory form.
Applications: Western blot validated (all three clones). Serum, plasma, and hepatocyte lysate are strong positive controls. For plasma quantitation, use RP1 (N-terminal) as capture and RP3 (C-terminal) as detection in sandwich ELISA formats.
Immunogen: Synthetic peptides corresponding to non-overlapping regions of human kallistatin (UniProt P29622).
Reactivity: Human confirmed; mouse and rat predicted based on sequence homology.
Additional Specifications
| Size | 100 µg |
|---|---|
| Target Protein | Kallistatin (SERPINA4) |
| UniProt ID | P29622 |
| Gene Symbol | SERPINA4 |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptides corresponding to non-overlapping regions of human kallistatin (UniProt P29622): N-terminal, reactive center loop (RCL), and C-terminal. Each clone targets a distinct functional region for orthogonal detection. |
| Conjugation | Unconjugated |
| Formulation | PBS, 0.02% sodium azide, 50% glycerol |
| Storage Conditions | -20°C long-term; 4°C short-term (≤1 month); avoid repeated freeze-thaw |
| Reactivity Species | Human |
| Validated Applications | Western Blot (WB) |
| Recommended Dilution | WB 1:1000 |
| Volume / Size | 100 µL |
| Proteinase Class | Serine Inhibitor |
| Purity (%) | >95% (affinity purified) |
| Publication Citations | 0 |
| Alternate Names | Kallikrein-binding protein · Peptidase inhibitor 4 · PI-4 · Serpin A4 |
| References (HTML) |
|
| Potential Cross-Reactivity | Mouse, Rat |
Validation imagery coming soon
Western blot validation figures for Kallistatin (SERPINA4) will be published here as they are produced in-house.
If you would like to see existing validation data for this antibody before publication, request a sample copy.
Also known as:
- Kallikrein-binding protein
- Peptidase inhibitor 4
- PI-4
- Serpin A4
- UniProt Consortium. (2023) UniProt: the Universal Protein Knowledgebase in 2023. Nucleic Acids Res. 51(D1):D523–D531.
- Rawlings ND, Barrett AJ, Thomas PD, Huang X, Bateman A, Finn RD. (2018) The MEROPS database of proteolytic enzymes, their substrates and inhibitors in 2017. Nucleic Acids Res. 46(D1):D624–D632.
Help us track citations using this antibody — email us your DOI when your paper is accepted.