Serine Proteinases: TMPRSS4
RP3-TMPRSS4
Rabbit Antibody to TMPRSS-4 (Transmembrane Serine Proteinase-4, mCAP2, Channel Activating Protease-2, Membrane-Type Serine Protease-2, MT-SP2); Catalytic domain
Size: 100 ug
Price: This product is sold through abcam. Adding this product to your cart will create shopping list with links to current abcam product information.
Description:
RP3-TMPRSS-4 is a rabbit polyclonal antibody made to the type-II transmembrane serine proteinase TMPRSS-4 (Transmembrane Serine Proteinase-4, mCAP2, Channel Activating Protease-2, Membrane-Type Serine Protease-2, MT-SP2)). The antibody is made to a synthetic peptide based on the catalytic domain of human TMPRSS-4. RP3-TMPRSS-4 reacts with human TMPRSS-4, and does not react with the other TMPRSSs. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.
Use:
TMPRSS-4 was first described as a trypsin-like serine proteinase overexpressed in pancreatic tumor cells, and named TMPRSS-3. This protein was later renamed TMPRSS-4, leading to some confusion in the literature. TMPRSS-4 is also known as Membrane-Type Serine Protease-2 (MT-SP2), and Channel Activating Protease-2 (mCAP2 is the mouse orthologue). The CAP-2 designation is also assigned to other proteases in the literature, but TMPRSS-4 is thought to process sodium channels in endothelial cells. TMPRSS-4 was also identified as overexpressed in thyroid neoplasms, and splice variants in TMPRSS4 are thought to be linked with different cancers. TMPRSS-4 is a member of the type-II transmembrane serine proteinases, such as Hepsin, Epithelisin, Corin, Matriptase and enteropeptidase. The TMPRSS-4 domain structure most resembles the TMPRSS-2 domain structure, and includes a cytoplasmic domain, a transmembrane domain, an LDL Receptor-like A domain, a SRCR (Scavenger Receptor-Cysteine Rich) domain (also called macrophage scavenger-like domain), and finally the trypsin-like serine proteinase domain. The human TMPRSS-4 sequences found to date encode proteins of 494, 492, 437, 435, 432, 423 and 335 amino acids. The predicted masses are 54.6, 54.4, 48.2, 48.0, 47.6, 46.4 and 37.2 kDa, respectively. Little is known about the relative expression of these different versions of TMPRSS-4. All but the 423 amino acid form start at roughly the same place (the 494, 437 and 432 forms start 2 residues earlier), and the 423 amino acid form has a different cytoplasmic domain than the other forms. There is also a 5 amino acid deletion in the 432 AA form, in the SRCR domain, relative to the other isoforms. The 335 AA form ends in the catalytic domain, and may not be proteolytically active. The 494 and 492 AA forms have 56 residue extensions to the carboxy end of the catalytic domain, relative to the other forms. Glycosylation and other posttranslational modifications make TMPRSS-4 run at higher than expected mass on reduced SDS PAGE gels. RP3-TMPRSS-4 recognizes all of the human TMPRSS-4 forms listed. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.
FOR RESEARCH USE ONLY; NOT FOR USE IN HUMANS.
Storage:
The undiluted antibody solution is stable for approximately 12 months at -20°C.
This product is sold through abcam.