 |
|
|---|---|
| Data Sheet for RP4TIMP1 | |
| Product Name | Rabbit Antibody toTIMP-1 (Tissue Inhibitor of Metalloproteinases) Loop #1 |
| Catalog Number | RP4TIMP1 |
| Product size | 100 ug |
| Description | RP4T1 is a Rabbit antibody to the Tissue Inhibitor of Metalloproteinases, TIMP-1, made with a synthetic peptide based on loop #1 in the Human sequence. The antibody binds to TIMP-1, but does not cross react with the other TIMP family members (TIMP-2, TIMP-3, TIMP-4). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant. |
Use
|
RP4T1 Anti-TIMP-1 works well for Western Blotting applications, and when used against the reduced protein identifies a band at 29 Kd. RP4T1 also binds to the non-reduced protein, and has use for immunoprecipitation, immunohistochemistry, and ELISA applications. TIMP-1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP-1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
Triple Point Biologics products are sold exclusively through Abcam. For availability, pricing and buying information please select your country below and press 'Go'. You will be relocated to the relevant datasheet page on Abcam's website. |
