 |
|
|---|---|
| Data Sheet for RP4MMP9 | |
| Product Name | Rabbit Antibody to Gelatinase-B (MMP-9); Propeptide region |
| Catalog Number | RP4MMP9 |
| Product size | 100 ug |
| Description | RP4MMP9 is a Rabbit antibody to the Matrix Metalloproteinase Gelatinase-B (92 Kd Gelatinase, MMP-9), made with a synthetic peptide based on the Propeptide region of the Human sequence. The antibody binds to Gelatinase-B, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc)The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant |
Use
|
RP4MMP9 recognizes the Propeptide region of MMP-9, which is shed after enzymatic activation. The antibody does not recognize the activated MMP-9, thus can be used to differentiate between latent and active MMP-9. RP4MMP9 anti-MMP9 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 92 Kd (the zymogen). The antibody recognizes both native and reduced forms of MMP-9, and can also be used for immunoprecipitation, immunohistochemistry, and ELISA applications. Gelatinase-B is constitutively produced by some tumor cell lines (e.g.: HT1080, HL60, U937) but not by most quiescent cells and tissues. Treatment of cells with the phorbol ester TPA stimulates production of MMP-9 in some cell types, but the low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western blotting. Gelatinase-B and Gelatinase-A may be concentrated by binding to gelatin-agarose, and eluted with 10% DMSO. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
Triple Point Biologics products are sold exclusively through Abcam. For availability, pricing and buying information please select your country below and press 'Go'. You will be relocated to the relevant datasheet page on Abcam's website. |
