Use

Calpain-11 is an intracellular, calcium-dependent cysteine protease. Calpain-11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. The 702 amino acid sequence predicts a protein of 80.582 kD. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain-11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain-11 closest to chicken calpain u/m, and it may be that calpain-11 is the human orthologue of this protein. RP4Calpain-11 recognizes latent and the aminotruncated (active) Calpain-11. The latent large subunit migrates at about 81 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 kD form. A cascade of smaller forms can be seen with further activation. RP4Calpain-11 Anti Calpain-11 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 81 kD, and a series of further cleaved forms. RP4Calpain-11 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Not all cell types produce Calpain-11; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human Calpain-11 are detected with RP4Calpain-11. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Triple Point Biologics products are sold exclusively through Abcam. For availability, pricing and buying information please select your country below and press 'Go'. You will be relocated to the relevant datasheet page on Abcam's website.