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| Data Sheet for RP4ADAM17 | |
| Product Name | Rabbit Antibody to ADAM-17 (A Disintegrin And Metalloproteinase-17, TNF-a Converting Enzyme, TACE) ; Activation site |
| Catalog Number | RP4ADAM17 |
| Product size | 100 ug |
| Description | RP4ADAM-17 is a polyclonal antibody made to the metalloproteinase ADAM-17. The antibody is made to a synthetic peptide based on the activation site (cysteine switch and furin cleavage site) of human ADAM-17. RP4ADAM-17 reacts with human, porcine and rat ADAM-17, and does not react with other ADAMs. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.01% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. |
Use
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also known as TNF-a Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein-rich domain, EGF-like repeat, type-I tyransmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), ADAM-17 is known to process the TNF-a trimer from the membrane-attached precursor to the soluble form. ADAM-17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L-selectin and TGF-a, making ADAM17 a "sheddase." ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM-17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different Cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other posttranslational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. RP4ADAM-17 recognizes a 120 kD band in reduced Western blots of conditioned media or cell lysates, which is converted to a 55-60 kD band. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
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