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| Data Sheet for RP3TIMP1 | |
| Product Name | Rabbit Antibody toTIMP-1 (Tissue Inhibitor of Metalloproteinases) Carboxyterminal region |
| Catalog Number | RP3TIMP1 |
| Product size | 100 ug |
| Description | RP3T1 is a Rabbit antibody to the Tissue Inhibitor of Metalloproteinases, TIMP-1, made with a synthetic peptide based on the carboxyterminal region of the Human sequence. The antibody binds to TIMP-1, but does not cross react with the other TIMP family members (TIMP-2, TIMP-3, TIMP-4). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant. |
Use
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RP3T1 Anti-TIMP-1 works well for Western Blotting applications, and when used against the reduced protein identifies a band at 29 Kd. RP3T1 recognizes both reduced and non-reduced protein, and has applications for immunoprecipitation, immunohistochemistry, and ELISA applications. TIMP-1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP-1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. RP3T1 recognizes a wider species range of TIMP-1 than does RP1T1 FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
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