ADAM-9, also known as MDC9 and Meltrin-gamma, was first described in muscle cells as a protein with homology to the fertilins (ADAMs 1, 2). Initial observations indicated a role for ADAM-9 in myoblast fusion, similar to sperm-egg fusion aided by ADAMs 1 and 2. Later works describe ADAM-9 in the lung, heart, brain, kidney, and a wide range of tissues, and examined the ability of ADAM-9 to activate TNF-? and HB-EGF and to act as an adhesion molecule with integrin ?v?5. The Cytoplasmic domain of ADAM-9 contains SH3 ligand domains, which are thought to interact with PKC-?, suggesting specific regulation routes for ADAM-9. A novel form of ADAM-9 was reported in myeloma cells, with truncated amino and carboxyterminal ends, and a 13 amino acid insert in the cysteine-rich domain. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), the function of ADAM-9 is still poorly understood. ADAM-9 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM-9 sequence codes for an 819 amino acid protein, containing a Type-I transmembrane domain. The predicted mass is 90.5 kD, but glycosylation and cyteine-rich regions make the protein run at 114 kD in unprocessed, and 84 kD in processed forms. The Mouse ADAM-9 sequence is 845 AA, with a predicted mass of 92 kD. RP3-ADAM-9 recognizes bands at 116 kD (minor band), 66 kD (major band), and breakdown products at 55 kD and 34 kD in reduced Western blots of cell lysates. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Triple Point Biologics products are sold exclusively through Abcam. For availability, pricing and buying information please select your country below and press 'Go'. You will be relocated to the relevant datasheet page on Abcam's website.