Use

ADAM-8, also known as MS2 and CD156, was first described as a monocyte-specific protein. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the function of ADAM-8 is still poorly understood. The name CD156 was given to indicate that ADAM-8 is a leukocyte differentiation antigen, where it may play an important role. ADAM-8 has been shown to stimulate osteoclasts, and to be produced by bone marrow cultures stimulated by vitamin-D. ADAM-8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to posses proteolytic activity. Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. Although ADAM-8 was reported to be monocyte specific, it is expressed in a wide range of cells in culture. The sequence codes for an 826 amino acid protein, containing a Type-I transmembrane domain. Studies of recombinant ADAM-8 show that little or none is expressed on the cell surface; the majority is shed into the cell culture media as a 70 kD form, similar to ADAM-8 secreted into media of bone marrow cells. RP3ADAM-8 recognizes 75 kD and 60 kD bands in reduced Western blots. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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