Rabbit Antibody to Kallikrein-9(KLK-L3); Catalytic domain

RP2-hK9 is a rabbit polyclonal antibody made to the serine proteinase kallikrein-9 (hK9).  The antibody is made to a synthetic peptide based on the catalytic domain of human Kallikrein-9.  RP2-hK9 reacts with human Kallikrein-9, and does not react with the other human kallikreins (hK1-15).  The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

Kallikrein-9, also known as Kallikrein-Like 3 (KLK-L3), is a chymotrypsin-like serine proteinase. Kallikrein-9 was discovered as the locus for kallikreins on chromosome 19 was more fully mapped, and found by similarity to the other tissue kallikreins. Kallikrein-9 has been found in the ovary, thymus, testis, prostate, skin, breast and neuronal tissues, and is made by many cell lines in culture. Kallikrein-9 levels in breast cancer and uterine cancer patients have been reported to drop as the disease progresses, thus hK9 might be considered a favorable prognostic marker. Different splice variants of hK9 have been reported, although it is not yet known if they produce functional proteins. The full length Kallikrein-9 encodes for a 250 amino acid protein, with a predicted mass of 27.5 kDa and a pI of 7.53. The 234 amino acid form predicts a protein of 26 kDa with a pI of 9.76, and this quite basic pI might give the shorter form a very different function or localization. The shorter sequence also diverges before the catalytic serine residue, making it unlikely to be proteolytically active. Pre-pro-kallikrein-9 has the 17 amino acid signal sequence is removed before secretion, and the Pro-kallikrein-9 is activated to Kallikrein-9 by removal of the 5 amino acid propeptide domain. Glycosylation and other post-translational modifications make the Kallikrein-9 protein run more slowly on SDS PAGE gels, usually around 36 kDa. A range of different sized bands can be seen for Kallikrein-9 in different tissues and in cell culture media, ranging from 16-30 kDa, and likely representing different proteolytically processed forms. Endogenous inhibitors of Kallikrein-9 include kallistatin, protein-C inhibitor and α1-proteinase inhibitor, although Kallikrein-9 can be found complexed to a number of different proteinase inhibitors. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.  Higher concentrations of antibody may be needed for samples from more distantly related species.  

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for approximately 12 months at -20°C.

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