Rabbit Antibody to Kallikrein-7(Stratum corneum chymotryptic enzyme (SCCE), PRSS6); Catalytic domain  

RP2-hK7 is a rabbit polyclonal antibody made to the serine proteinase kallikrein-7 (hK7).  The antibody is made to a synthetic peptide based on the catalytic domain of human Kallikrein-7.  RP2-hK7 reacts with human Kallikrein-7, and does not react with the human kallikreins (hK1-15).  The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

Kallikrein-7, also known as stratum corneum chymotryptic enzyme (SCCE) and PRSS6, is a chymotrypsin-like serine proteinase. Originally described from human skin as a serine protease involved in shedding of skin cells and remodeling if the skin, SCCE was later identified as Kallikrein-7. Kallikrein-7 is found at the highest levels in the skin, often complexed with the endogenous serpins SLPI or elafin, and kallikrein-7 can be found complexed to a number of different proteinase inhibitors. In addition to skin, Kallikrein-7 has been found in the kidney, esophagus, neuronal tissues, amniotic fluid, saliva, breast milk, urine, synovial fluid, seminal plasma and serum. Kallikrein-7 has been reported to be decreased in the CSF of Alzheimer’s patients, and message levels of KLK7 were decreased in adenocarcinoma. In skin, overexpression of hK7 has been shown to cause a form of dermatitis, and in psoriasis hK7 is expressed in higher levels than controls. The skin adhesive proteins corneodesmosin and desmocollin 1 have been reported to be substrates of Kallikrein-7, as is interleukin-1β and the insulin B-chain. Splice variants of Kallikrein-7 have been suggested as markers in ovarian cancer, and hK7 is reported to be elevated in squamous cervical cancer cells. Kallikrein-7 gene expression has been reported to be hormonally regulated, increased by estrogen in breast cancer cells in culture. Pre-pro-kallikrein-7 has the 22 amino acid signal sequence is removed before secretion, and the Pro-kallikrein-7 is activated to Kallikrein-7 by removal of the 7 amino acid propeptide domain. The full length Kallikrein-7 sequence codes for a protein of 253 amino acids, with predicted mass of 27.6 kDa, and a pI of 9.49. A shorter sequence starting at the second methionine has been reported, the 181 amino acid protein has a predicted mass of 19.9 kDa and a pI of 9.67. This shorter sequence, if produced as a protein, would lack the cysteine of the catalytic triad, and likely be proteolytically inactive. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.  Higher concentrations of antibody may be needed for samples from more distantly related species.  

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for approximately 12 months at -20°C.

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