Rabbit Antibody to Kallikrein-6 (Zyme, Neurosin, PRSS9, Protease M, Myelencephalon-specific protease (MSP)); Catalytic domain

RP2-hK6 is a rabbit polyclonal antibody made to the serine proteinase kallikrein-6 (hK6).  The antibody is made to a synthetic peptide based on the catalytic domain of human Kallikrein-6.  RP2-hK6 reacts with human Kallikrein-6, and does not react with the human kallikreins (hK1-15).  The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

Kallikrein-6, also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikrein-6 was originally described from the brain as an enzyme involved in degradation of amyloid plaque protein (APP), and hK6 was thought to be a beta secretase. Kallikrein-6 was shown to be elevated in the sera patients with Alzheimer’s disease and Parkinson’s disease, and in animal models of multiple sclerosis. The neuronal protein alpha-synuclein was shown to be cleaved by hK6, as has APP, and hK6 has been implicated in vascular morphogenesis and angiogenesis. MSP has also been shown to rapidly degrade myelin-specific protein, laminin and fibronectin. Kallikrein-6 is found is greatest abundance in the brain and neuronal tissues, with lesser levels in the kidney, thymus, spleen and lymph nodes. Human breast milk is reported to contain high levels of Kallikrein-6, and hK6 has bee purified from cerebral spinal fluid (CSF). Less stringent PCR detects hK6 in many tissues, and analysis of cell lines by Western blot shows that many cell lines make hK6. Endogenous inhibitors include kallistatin, protein-C inhibitor and α1-proteinase inhibitor, although Kallikrein-6 can be found complexed to a number of different proteinase inhibitors. Pre-pro-kallikrein-6 has the 16 amino acid signal sequence that is removed before secretion, and the Pro-kallikrein-5 is activated to Kallikrein-5 by removal of the 5 amino acid propeptide domain. The full length hK6 sequence codes for a protein of 244 amino acids, with predicted mass of 26.9 kDa, and a pI of 7.53, and the processed hK6 has an apparent mass of 31-33 kDa on reduced SDS PAGE gels.  A range of different sized bands can be seen for Kallikrein-6 in different tissues and in cell culture media, ranging from 16-32 kDa, and likely representing different proteolytically processed forms, but perhaps also the different splice variants. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.  Higher concentrations of antibody may be needed for samples from more distantly related species.  

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for approximately 12 months at -20°C.

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