Rabbit Antibody to Kallikrein-2 (Glandular Kallikrein); Catalytic domain

RP2-hK2 is a rabbit polyclonal antibody made to the serine proteinase kallikrein-2 (hK2).  The antibody is made to a synthetic peptide based on the catalytic domain of human Kallikrein-2.  RP2-hK2 reacts with human Kallikrein-2, and does not react with the human kallikreins (hK1, 3-15).  The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

Kallikrein-2, also known as human glandular kallikrein (hGK-1), was initially cloned from a human genomic library, and later isolated from prostate tissue. Kallikrein-2 is most closely related to Kallikrein-3 (PSA), with 78% identity, and with kallikrein-1, at 68% identity. Like Kallikrein-3, Kallikrein-2 is found in greatest abundance in the prostate, but at much lower levels than Kallikrein-3. In addition, Kallikrein-2 seems to be more labile than Kallikrein-1 or Kallikrein-3, and is often found in a cleaved form. Kallikrein-2 is inhibited by Protein C Inhibitor (PCI), and has been reposted as 55 kDa and 75 kDa complexes with this inhibitor. The prostate tumor cell line LNCaP produces Kallikrein-2, along with kallikreins 1 and 3, and the kallikreins are found in the conditioned media as 33, 20 and 15 kDa bands on SDS PAGE gels. Others have isolated a 31 kDa band from seminal plasma, with an amino sequence of IVGGWE, indicating the mature active enzyme. The free cleaved Kallikrein was shown to be a triplet at around 22 kDa. A 62 kDa band was also detected in prostate carcinoma cells, and an 85 kDa band in seminal plasma, perhaps indicating different forms bound to inhibitors. Kallikrein-2 has also been detected in human serum, with levels elevated in prostate cancer and benign hyperplasia, and at low levels in human breast milk and saliva. Purified kallikrein-2 has been shown to bind to protease Inhibitor-6 (PI-6), PAI-1, α1-antichymotrypsin (α1-ACT), α2-antiplasmin (α2-AP), antithrombin III (AT-III) and α-2 MacroglobulinA (α2-M) in vitro, each generating different high molecular weight complexes. A polymorphic form of Kallikrein-2 (Trp226-hK2) is thought to have altered catalytic activity relative to wild type Kallikrein-2. Different messages for Kallikrein-2 have been discovered thus far, with different carboxyterminal truncations, but whether these different isoforms have different function or distribution is yet to be shown.  A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.  Higher concentrations of antibody may be needed for samples from more distantly related species.  

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for approximately 12 months at -20°C.

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