Rabbit Antibody to Kallikrein-14 (KLK-L6); Catalytic domain  

RP2-hK14 is a rabbit polyclonal antibody made to the serine proteinase kallikrein-14 (hK14).  The antibody is made to a synthetic peptide based on the catalytic domain of human kallikrein-14.  RP2-hK14 reacts with human kallikrein-14, and does not react with the human kallikreins (hK1-15).  The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

Kallikrein-14, also known as kallikrein-like6 (KLK-L6), is a trypsin-like serine proteinase. Kallikrein-14 was discovered by cloning the kallikrein locus on chromosome 19, and was found to be similar to the other kallikrein family genes. Kallikrein-14 is produced by a number of tissues, including the brain, spinal cord, esophagus, salivary gland, breast, prostate, uterus, lung, thymus and thyroid. Like many of the kallikreins, Kallikrein-14 is also found in biological fluids like serum, breast milk, amniotic fluid, seminal plasma and cerebrospinal fluid. Kallikrein-14 message has been reported to be decreased in some breast cancers, and in ovarian cancers, similar to Kallikrein-10. Some cell lines express Kallikrein-14 in culture, and like many of the kallikreins, the production is stimulated by hormones. Recombinant kallikrein-14 was profiled on peptide substrates, and found to possess both trypsin-like and chymotrypsin-like activity. The ECM proteins lamanin and collagen-IV are thought to be substrates of Kallikrein-14. Endogenous inhibitors include kallistatin, protein-C inhibitor and α1-proteinase inhibitor, although Kallikrein-14 can be found complexed to a number of different proteinase inhibitors. The kallikrein-14 sequence codes for a protein of 251 amino acids, with predicted mass of 27.5 kDa, and a pI of 10.65. This quite basic pI likely influences the localization of Kallikrein-14. Glycosylation and other post-translational modifications make Kallikrein-14 run at a higher molecular weight on SDS PAGE gels. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.  Higher concentrations of antibody may be needed for samples from more distantly related species.  

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for approximately 12 months at -20°C.

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