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| Data Sheet for RP2TIMP3 | |
| Product Name | Rabbit Antibody toTIMP-3 (Tissue Inhibitor of Metalloproteinases 3); Carboxyterminal region |
| Catalog Number | RP2TIMP3 |
| Product size | 100 ug |
| Description | RP2T3 is a Rabbit antibody to the Tissue Inhibitor of Metalloproteinases, TIMP-3, made with a synthetic peptide based on the carboxyterminal region of the Human sequence. The antibody binds to TIMP-3, but does not cross react with the other TIMP family members (TIMP-1, TIMP-2, TIMP-4). RP2T3 recognizes the glycosylated and unglycosylated forms of TIMP-3, and works against native or reduced TIMP-3. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, 50% glycerol, with the addition of Sodium Azide as preservative. |
Use
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RP2T3 Anti-TIMP-3 works well for Western Blotting applications, and when used against the reduced protein identifies bands at approximately 24 Kd and 30 Kd (unglycosylated and glycosylated, respectively). RP2T3 recognizes both reduced and nonreduced TIMP-3. TIMP-3 is produced in low (pg/ml) levels by most cell types, and appears to be preferentially secreted into the extracellular matrix. For Western blots and reverse zymogram analysis, an ECM preparation is most often used, but a total cell lysate can also be used. A recommended starting concentration for Western blots is 1:1000 when using colorometric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
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