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| Data Sheet for RP2MMP18 | |
| Product Name | Rabbit Antibody to Xenopus Collagenase (Collagenase-4, MMP-18); Hinge region MMP-18 |
| Catalog Number | RP2MMP18 |
| Product size | 100 ug |
| Description | RP2MMP18 is a Rabbit antibody to the Matrix Metalloproteinase Collagenase -4(Xenopus Collagenase; MMP-18), made with a synthetic peptide based on the hinge region of Xenopus MMP-18. RP1MMP18 binds to all forms of MMP-18, latent and active. The antibody binds to Collagenase-4, but does not cross react with the other MMP family members (MMP-1, MMP2, MMP3, MMP9). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant. |
Use |
MMP-18 was first described in Xenopus during development, and is distinct from MMP-1, which it most closely resembles. MMP-18 has been shown to digest intact type-I collagen at the same site as the classical collagenases (MMP-1, MMP-8, MMP-13). RP2MMP18 Anti-MMP18 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the smaller activated forms. RP2MMP18 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA uses. Collagenase-4 is produced at very low levels in normal, quiescent Xenopus cells and tissues. Collagenase production can be induced by mitogens such as TPA or TNF-a in receptive cell types. Although MMP-18 has been reported in Xenopus tissues, no human orthologue has been identified to date. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP-1 is well conserved, higher concentrations of antibody may be needed for non-human samples. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
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