Rabbit Antibody to ADAMTS-8   (A Disintigrin And Metalloproteinase with ThromboSpondin motif-8,   METH-2);   Aminoterminal region

RP2-ADAMTS-8 is a polyclonal antibody made to the metalloproteinase ADAMTS-8.   The antibody is made to a synthetic peptide based on the aminotermianl region of human ADAMTS-8.   The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

ADAMTS-8, also known as METH-2, was first described as a protein elevated in human prostate tumor cells, as the human ortholog of the mouse protein METH-2.   Initial observations indicated a role for ADAMTS-4 in blocking angiogenesis, where ADAMTS-8 blocked FGF-2 induced angiogenesis in a corneal neovascularization model.   A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), the function of ADAMTS-8 is still poorly understood.   ADAMTS-8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active on a range of substrates.   ADAMTS-8 is inhibited by the endogenous MMP inhibitors, TIMP-1, -2, -3 and -4, but most efficiently by TIMP-3.   In addition to the metalloprotease domain, ADAMTS-8 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine-rich domain and thrombospondin-1 like domains.   ADAMTS-8 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein.   Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions.   The full length ADAMTS-8 sequence codes for a 890 amino acid protein, with a predicted mass of 96.671 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-8 a greater apparent molecular weight of about 110 kDa on reduced SDS-PAGE gels.     RP2-ADAMTS-8 recognizes the zymogen of ADAMTS-8 at 110 kDa in reduced Western blots, the truncated form at 67 kDa (major band), and breakdown products at 39 kDa in cell lysates. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.   Higher concentrations of antibody may be needed for samples from more distantly related species.   EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for approximately 6 months at   4 ° C.For longer storage, store at   -20 ° C.

Triple Point Biologics products are sold exclusively through Abcam. For availability, pricing and buying information please select your country below and press 'Go'. You will be relocated to the relevant datasheet page on Abcam's website.