Rabbit Antibody to ADAM-29 (A Disintegrin And Metalloproteinase-29); Propeptide domain

RP2-ADAM-29 is a polyclonal antibody made to the metalloproteinase ADAM-29.   The antibody is made to a synthetic peptide based on the amino end (propeptide) of human ADAM-29.   The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

ADAM-29 was first described almost simultaneously by two different groups that analyzed EST libraries for homology to other ADAMs proteins. Both groups identified several splice variants, the variations all being in the length of the cytoplasmic domain, and both found the messages limited to testis. Later, ADAM-29 message was shown to be elevated in chronic lympocytic leukemia (CLL), and is thought to be a useful marker for CLL. The ADAM-29 is thus not specifically limited to testis, but to date there are no publications on ADAM-29 protein expression or localization. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), the function of ADAM-29 is still poorly understood.   ADAM-29 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif; rather ADAM-29 contains a HHxxHxxxxxH sequence, and as a result is thought to be proteolytically inactive.   Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions.   The full length ADAM-29 sequence codes for a 820 amino acid protein, containing a propeptide domain, "catalytic" domain, disintegrin domain, cysteine-rich domain, Type-I transmembrane domain,   and   a cytoplasmic domain. The predicted mass of 92.75 kDa is likely increased by glycosylation.   Two shorter sequences have been reported: 787 and 766 amino acid sequences that differ at the carboxyterminal end, with predicted masses of 89.46 and 86.98 kDa respectively.   Mouse ADAM-29 sequence encodes a 763 AA protein, with mass predicted at 86.46 kDa. The Cytoplasmic domain of ADAM-29, like ADAMs 9, 12 and 15, contains SH3 ligand domains, which are thought to interact with PKC- ?, suggesting specific regulation routes for ADAM-29. ADAM-29 may also be shed from the cell surface by cleavage extracellular from the transmembrane domain, resulting in a soluble form. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.   Higher concentrations of antibody may be needed for samples from more distantly related species.  

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for 12 months at -20 ° C.

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