Rabbit Antibody to Kallikrein-8(Neuropsin, Ovasin, TADG-14, PRSS19, HNP); Kallikrein loop  

RP1-hK8 is a rabbit polyclonal antibody made to the serine proteinase kallikrein-8 (hK8).  The antibody is made to a synthetic peptide based on the Kallikrein loop area  of human Kallikrein-8.  RP1-hK8 reacts with human Kallikrein-8, and does not react with the human kallikreins (hK1-15).  The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

Kallikrein-8 (hK8), also known as Neuropsin, Brain Serine Protease-1 (BSP1), tumor-associated differentially expressed gene-14 (TADG-14), PRSS19, HNP and Ovasin is a trypsin-like serine proteinase. The name neuopsin is intended to indicate a neuronal trypsin-like proteinase, but a literature alias for a form of opsin-5, also called neuopsin, confuses things a bit.  Originally described from mouse brain, KLK8 was implicated in neural plasticity and learning. Kallikrein-8 is thought to be involved in neurite outgrowth and fasciculation during nervous system development. Kallikrein-8 is induced in neurite injury, and Kallikrein-8 has been shown to be elevated in the hippocampus on Alzheimer’s patients’ brains. Animal models of autoimmune encephalomyelitis (EAE) show elevated Kallikrein-8 production. Kallikrein-8 null mice have aberrant synapses in their hippocampus, compared to wild type mice, and are predisposed to seizures. Kallikrein-8 is thought to regulate the early phase of long-term potentiation (LTP), possibly by cleaving fibronectin and other substrates.Kallikrein-8 is also found in the uterus, where it is hormonally regulated. Cervical cancer cell lines express high levels of Kallikrein-8, and it may be useful as a tumor marker. Kallikrein-8 is not limited to the brain and uterus, as many tissues and cell lines make the protein. Endogenous inhibitors include serine proteinase inhibitor-3, protein-C inhibitor and α1-proteinase inhibitor, although Kallikrein-8 can be found complexed to a number of different proteinase inhibitors. A number of different splice variants and partial sequences have been reported for Kallikrein-8, the longest encodes a protein of 305 amino acids with a predicted mass of 32.97 kDa and a pI of 8.59. The 260 amino acid form has a predicted mass of 28 kDa and pI of 8.18, the 164, 134 and 119 amino acid versions 17.9, 14.7 and 12.7 kDa respectively, but it is unclear if these proteins are produced. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.  Higher concentrations of antibody may be needed for samples from more distantly related species.  

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for 12 months at -20°C.

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