Kallikrein-1, also known as tissue kallikrein, glandular kallikrein, pancreatic kallikrein, renal kallikrein, urinary kallikrein and hPRK, is a trypsin-like serine proteinase. Originall described from human urine as a hypotensive agent, and later from the pancreas, kallkreins were given the Greek name for pancreas, kallikreas. Kallikrein-1 releases lysly-bradykinin from L-kininogen, and the pH optimum of the enzyme is 8.5-9.0. Kallikrein-1 is most closely related to Kallikrein-2 (glandular Kallikrein) and Kallikrein-3 (PSA), the three genes comprising the gtissue Kallikreinh family, and more distantly to the other human kallikreins (4-15). Endogenous inhibitors include kallistatin, protein-C inhibitor and ƒ¿1-proteinase inhibitor, although Kallikrein-1 can be found complexed to a number of different proteinase inhibitors. Pre-pro-kallikrein-1 has the 17 amino acid signal sequence is removed before secretion, and the Pro-kallikrein-1 is activated to Kallikrein-1 by removal of the 7 amino acid propeptide domain. The full length Kallikrein sequence codes for a protein of 262 amino acids, with predicted mass of 28.92 kDa, and a pI of 4.54, but extensive glycosylation and other posttranslational modifications give Kallikrein an apparent mass of 36-46 kDa on SDS PAGE gels, and a more acidic pI of 3.9-4.0. A range of different sized bands can be seen for Kallikrein-1 in different tissues and in cell culture media, ranging from 16-38 kDa, and likely representing different proteolytically processed forms. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Triple Point Biologics products are sold exclusively through Abcam. For availability, pricing and buying information please select your country below and press 'Go'. You will be relocated to the relevant datasheet page on Abcam's website.