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| Data Sheet for RP1MMP14 | |
| Product Name | Rabbit Antibody to Membrane-type Matrix Metalloproteinase-1 (MT1-MMP, MMP-14), Hinge region |
| Catalog Number | RP1MMP14 |
| Product size | 100 ug |
| Description | RP1MMP14 is a Rabbit antibody to the membrane-bound Matrix Metalloproteinase-1 (MMP-14), made with a synthetic peptide based on the Human sequence. The antibody binds to the hinge region of MT1MMP. The antibody binds to MT1MMP, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP-9). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative, and 50% glycerol as cryoprotectant. |
Use |
RP1MMP14 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 65 Kd and 63 Kd (the pro-form and active form), as well as activation/breakdown products. The antibody can recognize native or reduced MT1MMP, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. MT1MMP has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines. Treatment of cells with Concanavolin-A or the phorbol ester TPA stimulates production of MT1MMP in some cell types, and the enzyme can be recovered in cell lysates. Shed forms of MT1MMP have also been reported. A recommended starting concentration for Western blots is 1:2,000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
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