Rabbit Antibody to ADAMTS-8   (A Disintigrin And Metalloproteinase with ThromboSpondin motif-8,   METH-2);   Carboxyterminal end

RP1-ADAMTS-8 is a polyclonal antibody made to the metalloproteinase ADAMTS-8.   The antibody is made to a synthetic peptide based on the carboxyterminal end of human ADAMTS-8.   The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.

ADAMTS-8, also known as METH-2, was first described as a protein elevated in human prostate tumor cells, as the human ortholog of the mouse protein METH-2.   Initial observations indicated a role for ADAMTS-4 in blocking angiogenesis, where ADAMTS-8 blocked FGF-2 induced angiogenesis in a corneal neovascularization model.   A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), the function of ADAMTS-8 is still poorly understood.   ADAMTS-8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active on a range of substrates.   ADAMTS-8 is inhibited by the endogenous MMP inhibitors, TIMP-1, -2, -3 and -4, but most efficiently by TIMP-3.   In addition to the metalloprotease domain, ADAMTS-8 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine-rich domain and thrombospondin-1 like domains.   ADAMTS-8 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein.   Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions.   The full length ADAMTS-8 sequence codes for a 890 amino acid protein, with a predicted mass of 96.671 kD, but glycosylation and the abundance of cysteine residues gives ADAMTS-8 a greater apparent molecular weight of about 110 kD on reduced SDS-PAGE gels.   RP1-ADAMTS-8 recognizes the zymogen of ADAMTS-8 at 110 kD in reduced Western blots, the activated form at 64 kD (major band), and breakdown products at 30 kD of cell lysates. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.   Higher concentrations of antibody may be needed for samples from more distantly related species.   EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

The undiluted antibody solution is stable for approximately 6 months at   4 ° C.For longer storage, store at   -20 ° C.

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