 |
|
|---|---|
| Data Sheet for RHMMP2 | |
| Product Name | Rabbit Antibody to Gelatinase-A (MMP-2); Whole molecule |
| Catalog Number | RHMMP2 |
| Product size | 100 ug |
| Description | RHMMP2 is a Rabbit antibody to the Matrix Metalloproteinase Gelatinase-A (72 Kd Gelatinase, MMP2), made to native human MMP-2. The antibody binds to Gelatinase-A, but does not cross react with the other MMP family members (MMP-1, MMP-3, MMP-9, etc.). The antibody has been Protein-G affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant |
Use |
RHMMP2 Anti-Gelatinase-A works well for Western Blotting applications, and when used against the reduced protein identifies bands at 72 Kd and 68 Kd (the pro-form and active form). The antibody is made against native human MMP-2, and is protein-G purified. RHMMP2 binds both native and reduced forms of MMP-2, and may also be used for immunoprecipitation, immunohistochemistry, and ELISA applications. Gelatinase-A is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western Blotting. Gelatinase-A and Gelatinase-B may be enriched from conditioned culture media by binding to gelatin-agarose, and eluted by 10% DMSO. The recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
Triple Point Biologics products are sold exclusively through Abcam. For availability, pricing and buying information please select your country below and press 'Go'. You will be relocated to the relevant datasheet page on Abcam's website. |