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| Data Sheet for MABTIMP1 | |
| Product Name | Mouse Mouse monoclonal Antibody to TIMP-1 (Tissue Inhibitor of Metalloproteinases) Clone 2E7.1 Antibody to Stromelysin-1 (MMP-3) |
| Catalog Number | MABTIMP1 |
| Product size | 100 ug |
| Description | Mab-TIMP-1 is a mouse monoclonal antibody to the Tissue Inhibitor of Metalloproteinases, TIMP-1, made with native human TIMP-1. The antibody binds to TIMP-1, but does not cross react with the other TIMP family members (TIMP-2, TIMP-3, TIMP-4). The antibody has been affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant. |
Use |
Mab-TIMP-1 Anti-TIMP-1 works well for Western Blotting applications, and when used against the reduced protein identifies a band at 29 Kd. M-TIMP-1 recognizes both native and reduced TIMP-1, and has applications for immunoprecipitation and immunohistochemistry. For ELISA applications, the Mab-TIMP-1 may be paired with our rabbit anti-TIMP-1 antibodies. TIMP-1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP-1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, mix antibody solution with glycerol 1:1 to prevent freezing and store at -20°C. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
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