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| Data Sheet for MABMMP1 | |
| Product Name | Mouse monoclonal Antibody to Interstitial Collagenase (MMP-1) |
| Catalog Number | MABMMP1 |
| Product size | 100 ug |
| Description | Mab-MMP-1 is a Mouse monoclonal antibody to the Matrix Metalloproteinase Collagenase (Interstitial Collagenase; MMP-1), made with native human MMP-1. The antibody binds to Collagenase, but does not cross react with the other MMP family members (MMP-2, MMP-3, MMP-9, etc.). The antibody has been affinity purified, concentrated to 1-3 mg/ml, with the addition of Sodium Azide as preservative. |
Use |
Mab-MMP-1 Anti-Collagenase works well for Western Blotting applications, and when used against the reduced protein identifies bands at 53 Kd and 51 Kd (the pro-form), as well as active forms. Mab-MMP-1 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and can be paired with our rabbit anti-MMP-1 antibodies for ELISA. Collagenase levels in quiescent cells and tissues is minimal, and stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of Collagenase produced. A recommended starting concentration for Western blots is 1:1000 when using colorometric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP-1 is well conserved, higher concentrations of antibody may be needed for non-human samples. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Storage | The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, mix antibody solution with glycerol 1:1 to prevent freezing and store at -20°C. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative. |
Ordering |
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