Data Sheet for HMMP7
Product Name Human MMP-7 (Matrilysin-1), purified enzyme
Catalog Number HMMP7
Product size 10 ug
Description H-MMP-7 is purified MMP-7 enzyme (Matrilysin-1). The enzyme is purified from human kidney cells stably transfected with full-length human MMP-7, and is essentially free of contaminating MMPs or TIMPs. The enzyme is supplied as a mixture of the zymogen and active enzyme, in a buffer of 10 mM MES pH 5.0, 0.250 M sodium chloride, 5 mM calcium chloride, 0.025% Brij-35, glycerol 50% (v/v), with 0.01% Sodium azide as preservative.
Use

H-MMP-7 is a member of the Matrix metalloproteinase family of endopeptidases, containing the canonical HExxHxxGxxH motif as a zinc binding active site. MMP-7 and MMP-26 (Matrilysin-2) are the only MMPs discovered that completely lack a hemopexin “regulatory domain” or hinge region. Like the other MMPs, MMP-7 is secreted as a zymogen, then activated. The 28 kD form is reduced to 18 kD by enzymatic cleavage after the conserved “cysteine switch.” MMP-7 is not constitutively produced, but is produced on demand in specific tissues. MMP-7 substrate specificity is broad, most closely resembling the activity of MMP-3 (Stromelysin-1). When MMP-7 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them. Uses of the purified enzyme include enzyme kinetics assays, EIA, Western blot and PAGE standards, and substrate assays.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Storage H-MMP-7, when stripped of TIMPs, is unstable, and must stored carefully. Avoid repeated freeze thaw cycles, and store the enzyme at -80°C in aliquots.