Data Sheet for HMMP11
Product Name Human MMP-11 (Stromelysin-3), purified enzyme
Catalog Number H-MMP-11
Product size 100 ug
Description H-MMP-11 is purified MMP-11 enzyme (Stromelysin-3). The enzyme is purified from stimulated human fibroblasts, and is free of contaminating MMPs or TIMPs. The enzyme is supplied as a mixture of the zymogen and active enzyme, and Western blotting detects the 60 kD zymogen as well as 50 kD activated enzyme. H-MMP-11 is supplied in a buffer of 10 mM Tris-HCl pH 7.4, 100 mM sodium chloride, 5 mM calcium chloride, 0.02% Brij-35, and 50% (v/v) glycerol.
Use

H-MMP-11 is produced by stimulated fibroblasts, and by a wide range of cell types. Originally thought to be a tumor marker protein, MMP-11 has since been found in fibroblasts stimulated by IL-1, and in arthritic tissues. MMP-11 has been reported to be elevated in some cell lines with retinoic acid treatment, and the regulation of MMP-11 may be different than the other classical MMPs. MMP-11 resembles MMP-3 (Stromelysin-1) somewhat, although MMP-11 has an insertion after the cysteine switch that is characteristic of the membrane-bound MMPs (MtMMMPs). MMP-11 also contains a furin cleavage site (RxKR) downstream from the cysteine switch, unlike Stromelysin-1 and Stromelysin-2, and is thought to be activated by Prohormone convertases such as furin. Unlike MMP-2 (type IV Collagenase, Gelatinase-A), MMP-11 is not usually constitutively produced. When MMP-11 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them. Uses of the purified enzyme include enzyme kinetics assays, EIA, Western blot and PAGE standards, and substrate assays.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Storage H-MMP-11, when stripped of TIMPs, is unstable, and must be stored carefully. Store the enzyme at -20°C.
Ordering
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